'Laboratory Protocol for Carbohydrates\r'

'For polysaccharide extract a. reprize procedure A. 2a †A. 2d with 10 mL of the polysaccharide extract from Expt. 6 precisely use 10 drops conc. HCl. B. General quizs for Carbohydrates evidence the ff. kale issues: 1% glucose, fructose, maltose, sucrose, lactose, agar-agar, gum arabic, glycogen, cotton, starch, polysaccharide solution from clams, and all hydrolysates from Part A. 1. Molisch examen a. Add 2 drops Molisch reagent to 1 mL sugar solution. Mix thoroughly. b. Incline the furnish and gently pour 3 mL conc H2SO4 down the side of the tube. c. vizor the pretension at the interface of the 2 layers. 2. Benedictss establish a.Add 1 ml of the solution to be tried to 5 ml of Benedicts solution, and crusade each tube. b. Place the tube in a turn water bathtub and erupt for 3 minutes. c. hold the tubes from the instigate and allow them to cool. d. strike out precipitation, if thither is any, and the simulation of the precipitate formed. 3. Barfoeds streaml et a. Add 1 ml of the solution to be probeed to 3 ml of freshly prompt Barfoeds reagent. b. Place test tubes into a change state water bath and heat for 3 minutes. c. Remove the tubes from the bath and allow to cool. Do not heat the tubes longer than 3 minutes, as a irresponsible test can be obtained with disaccharides if they are het up(p) long enough. 4. Lasker and Enkelwitz Test a.Add 1 ml of the solution to be well-tried to 5 ml of Benedicts solution in a test tube and flick well. b. high temperature the test tube in a 55 oC water bath for 20 minutes. c. Note changes after 10 mins and up to 20 mins. 5. Orcinol Test a. Add 1 ml of the solution to be tested to 3 ml of Orcinol reagent. b. Gently heat the tube to boiling. Allow the tube to cool. c. Note color of the solution or if there is any precipitate formed. 6. Mucic Acid Test a. Add 10 drops conc HNO3 to 3 ml of the solution to be tested and mix well. b. Heat on a boiling water bath until the volume of the solution is reduced to about 1 ml. c. Remove the mixture from the water bath and permit it cool in an ice bath. d. Note the formation of crystals, if any.Caution:  Perform the reception low a fume hood. 7. Iodine Test for Starch and Glycogen a. Add 2 drops of Lugols iodine solution to 10 drops of solution to be tested in a spot plate. b. Note color changes. Post-Lab Questions: 1. jibe the ends of the iodine test on the polysaccharides with their structures. 2. gibe the results of the tests on cotton with the structure of cellulose. 3. debate the balanced chemical equation, the positive result and the product/s responsible for the positive result of each color reaction test. 4. Conclude on the type of carbohydrate in your polysaccharide extract found on the results of its color reactions.\r\n'